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Publications

MOST RECENT PUBLICATIONS

Antibacterial potential of an ethnic probiotic containing food,
Akhuni/Axone and its chemicalcharacterization

  • Akhuni/Axoni is a popular traditional fermented soybean food of northeast India

  • The antibacterial potential of extracts of Akhuni/Axone was determined against bacterial pathogens.

  • Mechanism of antibacterial activity and chemical characterization (HPTLC, 1H NMR, 13C NMR and GC-MS) explained.

  • The study might increase the functional property as well as broaden the scope of this traditional food, like other fermented foods of the world.

Anti-viral triterpenes: a review.

Triterpenes are naturally occurring derivatives biosynthesized following the isoprene rule of Ruzicka. The triterpenes have been reported to possess a wide range of therapeutic applications including anti-viral properties. In this review, the recent studies (2010–2020) concerning the anti-viral activities of triterpenes have been summarized. The structure activity relationship studies have been described as well as brief biosynthesis of these triterpenes is discussed.

Citrus macroptera induces apoptosis via death receptor and
mitochondrial mediated pathway as prooxidant in human
non-small cell lung cancer cells

The role of plant-based prooxidants as the selective anticancer agents is of a great promise. Citrus macroptera commonly known as “Satkara”, is an important herbal and medicinal plant native to Southeast Asia. In this study, the antiproliferative effect of ethyl acetate extract of C. macroptera peel (CPEA) was demonstrated on human non-small cell lung cancer cells through prooxidant induced apoptosis. Fatty acids, eight coumarins (three furanocoumarins) and one triterpenoid were identified from the CPEA through rigorous spectroscopic (UV, 1H and 13C NMR) and spectrometric (GC-MS, LC-ESI-HRMS) studies.

Development of a peptide-based bifunctional chelator conjugated to a cytotoxic drug for the treatment of melanotic melanoma

​

The cytotoxic drug gemcitabine (GEM) has been conjugated to receptor-binding peptides to target melanoma tumors. A hexapeptide having a Lys–Gly–His–Lys sequence (pep-1), an octapeptide with an Arg–Gly–Asp–Lys–Gly–His–Lys sequence (pep-2), a GEM-conjugated Lys–Gly–His–Lys peptide (GEM–pep-3) and a GEM-conjugated Asp–Gly–Arg peptide (GEM–pep-4) were synthesized and characterized. In vitro uptake of fluorescently labeled GEM–pep-3 and GEM–pep-4 on B16F10 cells was investigated. Fluorescence microscopy studies demonstrated significant uptake of GEM–pep-3 in the B16F10 mouse melanoma cell line.

Other Publications

Lipid nanocarrier-based
transport of docetaxel across the blood brain barrier

​Successful treatment of brain cancer remains a formidable challenge in neuroscience research due to sub-therapeutic permeation of conventional chemotherapeutics across the blood–brain barrier (BBB). By optimizing various conditions and process parameters, we developed a phospholipid based nanosize carrier (NL) encapsulating docetaxel (DTX) and investigated its BBB crossing potential, both qualitatively and quantitatively, in vivo. 

A curcumin-docetaxel co-loaded nanosuspension with increased anti-breast cancer activity was developed. Curcumin is a potential anticancer agent with p-glycoprotein (p-gp) inhibiting activity may be co-administered with docetaxel as a nanosuspension to enhance its anticancer effect by increasing the oral bioavailability and decreasing drug efflux.

Exploring the Potential of
99mTc(CO)3-labeled triazolyl
peptides for tumor diagnosis.

In recent years the authors have reported on 99mTc(CO)3-labeled peptides that serve as carriers for biomolecules or radiopharmaceuticals to the tumors. The pharmacophores were radiolabeled in high yields with [99mTc(CO)3(H2O)3]+ metal aqua ion, characterized for their stability in serum and saline, as well as in His solution, and found to be substantially stable. 

Ursolic acid loaded PLGA
nanoparticles : in vitro and in
vivo evaluation to explore tumor targeting ability on B16F10 melanoma cell lines.

Preparation, characterization
and biological evaluation of fac (M (CO) 3)+ labeled amino
carboxy ligands

Ursolic acid (UA), a pentacyclic triterpenoid extracted from plants, shows promising inhibitory effect in different tumor bearing cell lines. In the present study we fabricated UA loaded PLGA nanoparticles (UA-NPs) as the drug carrier and thoroughly evaluated in vitro and in vivo the differential tumor targeting effects of UA and UA-NPs in B16F10 melanoma cells.

Evaluation of 99m Tc(i)-
tricarbonyl complexes of
fluoroquinolones for targeting
bacterial infection.

The aim of this study was to develop 99mTc(CO)3-labeled fluoroquinolones as novel SPECT radiopharmaceuticals for imaging bacterial infection. Fluoroquinolones, e.g., ofloxacin (OFX), levofloxacin (LVX), lomefloxacin (LMX) and norfloxacin (NFX) were labeled with a fac-[99mTc(CO)3(H2O)3]+ precursor. Scintigraphy studies also showed a significant uptake in the infectious lesions suggesting that 99mTc(CO)3-fluoroquinolones might be useful as diagnostic agents for targeted delivery in bacterial infection

Pulmonary Delivery of
Voriconazole Loaded
Nanoparticles Providing A
Prolonged Drug Level in Lungs- A Promise For Treating Fungal Infection.

The objective of this study is to radiolabel various amino carboxy based chelating ligands with fac(M(CO)3)+ core, their physicochemical and biological characterization so that they can be used as bifunctional chelators and could be incorporated into biomolecules.  The chelates were pure as identified by the chemical analysis of the Re-congener. They were stable both in vitro and in vivo

Synthesis and Evaluation of
Technetium-99m Labeled
Bioreductive Pharmacophores
Conjugated with Amino Acids
and Peptides for Tumor Imaging.

Synthesis and Evaluation of
Technetium-99m Labeled
Bioreductive Pharmacophores
Conjugated with Amino Acids and Peptides for Tumor Imaging. Selective uptake and retention in tumor with encouraging tumor/muscle and tumor/blood ratio and significant cellular uptake of fluorescence-labeled-2-nitroimidazolyl RGD indicate the great potentiality of the pharmacophore for further evaluation as potential molecular imaging agent in cancer diagnosis.

99m Tc-labeling of ciprofloxacin and
nitrofurylthiosemicarbazone
using fac-[99mTc (CO) 3 (H2O) 3] core: evaluation of their efficacy as infection imaging agents

In the present study, PLGA nanoparticles containing the antifungal drug voriconazole was prepared and two best formulations were selected for further characterization and in vivo studies. The study reveals that pulmonary administration of nanoparticles containing voriconazole could be a better therapeutic choice even as compared to the iv route of administration of the free drug and/or the drug loaded nanoparticles.

The aim of this study was to radiolabel ciprofloxacin (Cip) and nitrofuryl thiosemicarbazone (NFT) with the fac-[99mTc(CO)3(H2O)3] + core and to evaluate the ability of the radiopharmaceuticals as tracers in detecting sites of infection. Cip and NFT were radiolabeled with the fac-[99mTc(CO)3(H2O)3] + core and characterized by RHPLC. y. During scintigraphic studies, infection sites appeared quite distinctly with 99mTc(CO)3-Cip and 99mTc(CO)3-NFT, comparable to the behaviour with 99mTc(V)O-Cip

Synthesis, characterization, and biological evaluation of
99m Tc(CO) 3 -labeled peptides for potential use as tumor targeted radiopharmaceuticals.

In this study, we report the synthesis of two tetrapeptides (Asp-Gly-Arg-His and Asp-Gly-Arg-Cys) and one hexapeptide [Asp-Gly-Arg-D-Tyr-Lys-His] by changing the amino acid sequence of the Arg-Gly-Asp motif. The peptides were synthesized in excellent yield and labeled using freshly prepared [99mTc(CO)3(H2O)3]+ intermediate. 

Tricarbonyl 99mTc(I) and
Re(I)–thiosemicarbazone
complexes: synthesis,
characterization and biological
evaluation for targeting bacterial infection.

Methyl, ethyl and phenyl nitrofuryl thiosemicarbazone ligands (1, 2 and 3 respectively) were radiolabeled with freshly prepared aqueous solution of a fac[99mTc(CO)3(H2O)3]+ precursor. Biodistribution studies of 99mTc(CO)3-labeled thiosemicarbazones in rats intramuscularly infected with S. aureus exhibited substantial in vivo stability of the complex and moderate accumulation at the site of focal infection.

Tricarbonyltechnetium(I) and
tricarbonylrhenium(I)
complexes of amino acids:
crystal and molecular structure of a novel cyclic dimeric Re(CO) 3 -amino acid complex comprised of the OON donor atom set of the tridentate ligand.

Radiolabeled complexes of monoamino polycarboxylic, polyamino monocarboxylic and thiol containing amino acid ligands were prepared from a fac-[99mTc(CO)3(H2O)3]+ precursor.The overall radiochemical yield was 94–98%. The complexes exhibited substantial in vitro and in vivo stability. The amino carboxylate ligand system formed well defined complexes with a fac-[M(CO)3(H2O)3]+ core and shows good promise in 99mTc(CO)3 tracer development.

Curcumin-docetaxel co-
loaded nanosuspension for
enhanced anti-breast cancer
activity.

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